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- What are the restriction enzymes and why are they so important in rDNA technology?
- What is intellectual property and what rights are available to protect it?
- What are the advantages of getting a patent?
- What are ‘biosensors’? How are biosensors used for environmental monitoring and bio-monitoring?
- What is biohydrometallurgy and biomineralization? How can microbes be used for extraction of metals and for inducting mineral deposits?
- Describe in detail, the role of biofertilizers and bioinsecticides in protecting the environment?
- What is metabolic engineering and how can it be used for overproduction of metabolites in plants?
- What do you understand by ‘protein engineering’? What is the difference between ‘protein engineering’ and ‘genetic engineering’?
- What are “Transgenic plants”? How do we obtain transgenic plants?
- What is ‘molecular farming’? How and which transgenic plants can be used as ‘bioreactors’ for producing important drugs and chemicals?
- Write short notes on: a) virus mediated gene transfer, b) microinjection, c) electroporation, d) particle gun method.
- What are cybrids? How are they produced? Discuss the use of cybrids in crop improvement programmes.
- What is micropropagation? Describe the technique of micropropagation and discuss the different possible uses.
- What is somaclonal variation? Discuss the role that they play in crop improvement.
- What is gene therapy? What are the limitations and prospects of its use in medicine.
- What is the difference between “molecular farming” and “molecular pharming”? What useful products can be obtained by using transgenic animals as “bioreactors”?
- What are GM foods? What precautions one should observe regarding the safety of GM foods?
- What are the genetic engineering strategies to create (a) edible vaccines, (b) herbicide tolerant plants?
- What is explant culture? What are the steps involved in explant culture?
- What is direct gene transfer? What are the methods of direct gene transfer?
- Write a short note on “bioethics in animal genetic engineering”?
- What is meant by plant regeneration? Give the different pathways of plant regeneration.
- What is “molecular breeding”? What types of markers are used in screening/selection?
- What are the essential features of a vector?
- Write a short note on FISH technique? How can FISH technique help in the detection of chromosomal abnormalities?
- What is “micro array technology?
- Write a short note on ‘genome sequencing project’ and why is it essential for sequencing a genome?
- What are the different types of sequences entered into the database?
- What are the different methods for small scale sequencing of genome?
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How can we use comparative CDNA hybridization micro array method to differentiate between the normal cell and cancerous cells?
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